Composite

Part:BBa_K3110047

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-15)
Revision as of 19:29, 18 October 2019 by Meghamariajacob (Talk | contribs)


Promoter RBS FimH 136KO-RPMrel

FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to RPMrel. We amplified the FimHRPMrel from this existing part, and to this, we ligated a strong constitutive promoter and strong RBS for greater expression. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None