Part:BBa_K3084000

Venus Yellow Fluorescent Protein (YFP), S-TIP37 codon optimised

This Venus YFP gene has been designed for optimal translational efficiency from the genome of bacteriophage S-TIP37 during infection of its host, Synechococcus sp. strain WH8109. The translation of this gene is the same as the Venus YFP gene (NCBI accession number: ACQ43942). To maximise translational efficiency during S-TIP37 infection, the codon distribution of the S-TIP37 capsid gene, putatively the most strongly expressed gene on the S-TIP37 genome, was applied to the Venus YFP gene.

Comparing the translational efficiency to other YFPs in the S-TIP37 expression system required an experimental setup that was too complicated for the scope of our summer iGEM project. However, the fluorescence of our codon optimised gene was successfully tested using the pET expression system in E. Coli. Proof of fluorescence is demonstrated in figure 1.

Optimal excitation wavelengths were determined by Sarkar et al. and found to be: 285 and 470 nm.

Usage and biology

This part is useful as a reporter, most suitable for expression during Synechococcus infection by phage S-TIP37.

References

[1] Sarkar, P., Koushik, S. V., Vogel, S. S., Gryczynski, I., & Gryczynski, Z. (2009). Photophysical properties of Cerulean and Venus fluorescent proteins. Journal of biomedical optics, 14(3), 034047. doi:10.1117/1.3156842