Part:BBa_K3040014
pFadD promoter with pLac regulating downstream RFP
pFadD promoter is one of the regulator in the enzymes of fatty acid biosynthesis in E. coli. It is composed of two fadR recognition sites, and one CRP binding site. The fatty acid metabolism system of E. coli is consist of many parts, fadD, fadL, fadR, fadA, etc. Interestingly, each enzyme in this family has its own different sequence of fadR recognition site in its promoter, which makes these promoter having different strength, yet can still be interchangeable for us to apply in our system. Based on this fact, we tried pFadD promoter to take place of the weak pFadBA promoter. With two fadR recognition sites native in pFadD, we assume a better result in the sensitivity and a lower leakage in our pFadD promoter. Yet, we further modified pFadD promoter by replacing its CRP binding site with a FadR recognition site of the sequence of pFadBA promoter.
Reference
FENG, Youjun; CRONAN, John E. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PloS one, 2012, 7.9: e46275.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 30
Illegal suffix found in sequence at 230 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 30
Illegal SpeI site found at 231
Illegal PstI site found at 245
Illegal NotI site found at 36
Illegal NotI site found at 238 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 30
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 30
Illegal suffix found in sequence at 231 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 30
Illegal XbaI site found at 45
Illegal SpeI site found at 231
Illegal PstI site found at 245
Illegal AgeI site found at 863
Illegal AgeI site found at 975 - 1000COMPATIBLE WITH RFC[1000]
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