Composite

Part:BBa_K2963009

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-08)
Revision as of 14:57, 18 October 2019 by GHX (Talk | contribs)


capB*CA - encoding poly-γ-glutamic acid synthetase

CapBCA complex, consisting of three subunits, is composed of CapB、CapC and CapA. CapB catalyzes poly-gamma-Glutamic acid synthesis．And CapC links CapB and CapA in the membrane. While CapA transports poly-gamma-Glutamic acid outside the cell.In our project, we use the CapBCA complex to biosynthesize poly-gamma-Glutamic acid.

Usage and Biology

The BCA genes from Bacillus sp., encoding a polyglutamate synthetase located on the cell membrane, is capable of polymerizing glutamic acid to form poly-γ-glutamic acid. In Bacillus licheniformis, BCA are called capBCA.This part is for producing L-glutamate-rich γ-PGA. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NheI site found at 2365
    Illegal NheI site found at 2963
    Illegal NheI site found at 4281
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NgoMIV site found at 2590
  • 1000
    COMPATIBLE WITH RFC[1000]


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