![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3040007
Designed by: TSAI, CHIA-TSEN Group: iGEM19_NTHU_Taiwan (2019-10-18)
placUV5-R0010-TesA_pFadBA- RBS -mRFP
Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 1106
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1107
Illegal PstI site found at 1121
Illegal NotI site found at 1114 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1172
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 1107
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1107
Illegal PstI site found at 1121
Illegal AgeI site found at 1829
Illegal AgeI site found at 1941 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |