Composite

Part:BBa_K3096040

Designed by: Patrick Mueller, Famke Baeuerle, Lina Widerspick, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)
Revision as of 13:54, 18 October 2019 by PaMue (Talk | contribs) (→‎Sequence and Features)

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Temperature responsive repression system

A constitutively active promoter expresses Clts, a temperature sensitive cI repressor of the lambda phage. If the temperature is significantly below 37°C (body temperature), Clts is an active repressor for the cI lambda promoter. This promoter controls the transcription of a repressor protein, AraC. Therefore, if conditions are not 37°C, the AraC is not available, hence the pBAD promoter, controlled by AraC, is active and genes controlled by the pBAD promoter are transcribed. Accordingly, if the temperature is at 37°C, Clts is unstable and cannot repress the expression of AraC. AraC consequently inhibits the pBAD promoter and downstream genes are not expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

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Categories
Parameters
None