Composite
Part:BBa_K3040005
Designed by: TSAI, CHIA-TSEN Group: iGEM19_NTHU_Taiwan (2019-10-18)
Description
It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region. According to the previous research [1], there is improvement in expression for pFadBA with consensus sequence.
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Categories
Parameters
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