Regulatory
Part:BBa_K2934002:Design
Designed by: Shira Levi Group: iGEM19_Technion-Israel (2019-10-10)
pKatA promoter for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Suitable for Bacillus subtilis.
Primers for isolation of the gene (with RFC[10] suffix and prefix):
fwd: 5'-GGCCGCTTCTAGAGATAACTATTTT-3'
rev: 5'-CGCTACTAGTATAAGAACATAATATCATCTC-3'
Source
The sequence was taken from Herbig et al. 2001 [1].
References
[1] Andrew F. Herbig and John D. Helmann. 2001. Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA, Molecular Microbiology.