Coding

Part:BBa_K3228040

Designed by: Marian Krämer   Group: iGEM19_Marburg   (2019-10-14)
Revision as of 13:25, 18 October 2019 by MauriceMager (Talk | contribs)


pMC_0_4_22_NanoLuc-K1159001

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 520
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

specific text here

This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. Cyanobacteria show a different absorption and excitation behaviour than other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set is a recommendation of reporters based on experimental data LLLLink to experiments with cyanosLLL and literature.

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to .
Be aware that some of these reporters are codon optimized for Synechococcus elongatus UTEX2973.

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Categories
Parameters
None