Composite

Part:BBa_K3202029

Designed by: Heyuan Ni   Group: iGEM19_BHSF_ND   (2019-10-14)
Revision as of 13:06, 18 October 2019 by AndyXiong (Talk | contribs)


AraC-Pc-pBAD-PhiC31

This composite part is designed as a genetic circuit that is used as a control group of our later experiment. In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the pC-Xyls is used as a reference of what the bacterial colony should be like is there is completely no leakage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2016
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 294
    Illegal SapI site found at 399
    Illegal SapI site found at 457
    Illegal SapI site found at 1998
    Illegal SapI.rc site found at 148


[edit]
Categories
Parameters
None