Composite
Part:BBa_K3202029
Designed by: Heyuan Ni Group: iGEM19_BHSF_ND (2019-10-14)
AraC-Pc-pBAD-PhiC31
This composite part is designed as a genetic circuit that is used as a control group of our later experiment. In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the pC-Xyls is used as a reference of what the bacterial colony should be like is there is completely no leakage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2181
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2016
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 294
Illegal SapI site found at 399
Illegal SapI site found at 457
Illegal SapI site found at 1998
Illegal SapI.rc site found at 148
[edit]
Categories
Parameters
None |