Coding
ADR CMYC 6

Part:BBa_K3038001

Designed by: Marine LAVAUD   Group: iGEM19_Poitiers   (2019-10-10)
Revision as of 12:45, 18 October 2019 by Mlavaud (Talk | contribs) (→‎Description)


Alcohol dehydrogenase - Geobacillus stearothermophilus_Cmyc and 6His tags_Cterminal


Description

Alcohol dehydrogenase, ADH or ADR C-term, is a BioBrick C-Myc and 6-His tagged in C-term.

ADR is a thermophilic NAD+ dependent alcohol dehydrogenase. This enzyme bears mainly an ethanol-dehydrogenase activity.

GenBank

ADR : GenBank: P42327
https://www.ncbi.nlm.nih.gov/protein/P42327

Protein Sequence

MEQKLISEEDLNSAVDHHHHHHVKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGD WPIKPKLPLIPGHEGVGIVVEVAKGVKSIKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDG GYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALKVSGARPGEWVAIYGIGGLGHIALQYAKAMG LNVVAVDISDEKSKLAKDLGADIAINGLKEDPVKAIHDQVGGVHAAISVAVNKKAFEQAYQSVKRGGTLV VVGLPNADLPIPIFDTVLNGVSVKGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFERMEKGKI NGRIVLKLKED

Reaction

Primary alcohol + NAD+ = Aldehyde + NADH + H+ Secondary alcohol + NAD+ = ketone + H+ + NADH EC:1.1.1.1

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Categories
//chassis/prokaryote/ecoli
Parameters
None