Part:BBa_K2915275

IPTG inducible promoter (T7) with RBS TAL2 6-His-Tag with TEV site

The TAL2 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas. These designed proteins are able to bind itself specifically onto a DNA fragment ( 5'CCGCTG3') of a Tubercolusis gene . The promoter T7 and RBS (BBa_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha bacteria. We also tested production of the protein and used the His-Tag to purify the protein and we tested the activity of our protein using a gel shift.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 246
  

Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This TAL2 exists with:

- with a His-tag and a TEV site

- with a promotor T7 and RBS (BBa_K525998) which is the regulator.