Composite

Part:BBa_K3102044

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-15)
Revision as of 12:27, 18 October 2019 by ChloeDOIZELET (Talk | contribs)

Electron transfer improvement (ACS, ZWF, GND, PGL)

This "electron transfer improvement" composite is composed of acetyl-CoA synthetase (ACS):BBa_K3102031 which is used to generate acetyl-coA by degrading acetate but also of G6P dehydrogenase (ZWF):BBa_K3102032, 6-phosphogluconate dehydrogenase (GND):BBa_K3102033 and 6-phosphogluconolactonase (PGL): BBa_K3102034 enzymes necessary for the oxidative pentose phosphate pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2933
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2933
    Illegal NotI site found at 2814
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3490
    Illegal XhoI site found at 408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2933
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2933
    Illegal NgoMIV site found at 2206
    Illegal NgoMIV site found at 2923
    Illegal NgoMIV site found at 5493
    Illegal AgeI site found at 837
    Illegal AgeI site found at 4245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3975


[edit]
Categories
Parameters
None