Composite

Part:BBa_K3102046

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-15)
Revision as of 12:25, 18 October 2019 by ChloeDOIZELET (Talk | contribs)


Electrons production (pflB, ACS, FDH, MDH)

This “Electrons production” composite part is composed of pyruvate formate-lyase (pflB: BBa_K3102037), acetyl-CoA synthetase (ACS: BBa_K3102038), NAD-dependent formate dehydrogenase (FDH: BBa_K3102039) as well as the malate dehydrogenase (MDH: BBa_K3102040).

The pflB oxidizes pyruvate and acetyl-CoA while ACS induces the transformation of acetate into acetyl-CoA. Additionally, FDH and MDH lead to generation of cofactors and the oxidation of malate into oxaloacetate. Those are responsible for the production of reduced coenzymes generating electrons and all genes are overexpressed to enhance these mechanisms.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5516
    Illegal AgeI site found at 534
    Illegal AgeI site found at 3413
    Illegal AgeI site found at 6024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 836


[edit]
Categories
Parameters
None