Coding

Part:BBa_K2915225

Designed by: ARRINELL Jennifer   Group: iGEM19_Aix-Marseille   (2019-10-11)
Revision as of 12:21, 18 October 2019 by Jennyarrindell (Talk | contribs)


IPTG inducible promoter (T7) with RBS TAL1 6-His-Tag with TEV site

The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes This biobrick is in RFC 10 standards with: the prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3' This TAL1 exists with: - with a His-tag and a TEV site - with a promotor T7 and RBS (BBa_K525998) which is the regulator.

[edit]
Categories
Parameters
None