Device

Part:BBa_K2936009

Designed by: Jiang Weiyan   Group: iGEM19_ZJUT-China   (2019-10-09)
Revision as of 11:11, 18 October 2019 by Jiangweiyan (Talk | contribs)


light-controlled repressible system

This part contains a light-controlled system and a repression system to achieve downstream gene expression under light conditions rather than working in dark conditions. In this system, under dark conditions, phosphorylated FixJ protein was transferred from YF1 protein to FixJ protein, and phosphorylated FixJ protein activated pFIXK2 promoter, then activated lacI protein expression. When the LacI protein binds to LacO, it prevents the downward expression of Lac promoter, the target gene is not activated. When cultured under light conditions, phosphorylation of FixJ protein was blocked and gene expression regulated by pfixk2 promoter was inhibited. Lac promoter expression was not inhibited, target gene expression was normal. If you want to use this system,you just need to link the target gene to the lactose operon gene and then introducing plasmid into the host cell.After that,If the transformant is successfully selected, it is possible to express the target gene by illumination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


[edit]
Categories
Parameters
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