Composite

Part:BBa_K2921650

Designed by: Allison Kuo   Group: iGEM19_TAS_Taipei   (2019-10-14)
Revision as of 10:47, 18 October 2019 by Murpdurp888 (Talk | contribs)


Promoter + RBS + 6xHis + OprF + GS-linker + mRFP + Double Terminator

This construct constitutively expresses a colored metal-binding fusion protein: OprF linked with mRFP.

T--TAS_Taipei--lqK2921650.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 698
    Illegal AgeI site found at 1286
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None