Composite

Part:BBa_K3146012

Designed by: Milva Khandakar   Group: iGEM19_Westminster_UK   (2019-10-18)
Revision as of 10:13, 18 October 2019 by Milva98 (Talk | contribs)


RhlA with promoter and RBS

This is a composite part which Includes a promoter (BBa_J23100), RBS (BBa_B0034) and RhlA (BBa_K3146007)

Required for rhamnolipid surfactant production. Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta-hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. Rhamnolipid production plays an important role in swarming motility.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 130
    Illegal BamHI site found at 690
    Illegal XhoI site found at 866
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355
    Illegal BsaI.rc site found at 539


[edit]
Categories
Parameters
None