Composite
Part:BBa_K3146012
Designed by: Milva Khandakar Group: iGEM19_Westminster_UK (2019-10-18)
RhlA with promoter and RBS
This is a composite part which Includes a promoter (BBa_J23100), RBS (BBa_B0034) and RhlA (BBa_K3146007)
Required for rhamnolipid surfactant production. Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta-hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. Rhamnolipid production plays an important role in swarming motility.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 130
Illegal BamHI site found at 690
Illegal XhoI site found at 866 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 355
Illegal BsaI.rc site found at 539
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Categories
Parameters
None |