Composite

Part:BBa_K2921150

Designed by: Allison Kuo   Group: iGEM19_TAS_Taipei   (2019-10-14)
Revision as of 09:24, 18 October 2019 by Murpdurp888 (Talk | contribs)


6xHIS+LC Cutinase+GS+amilCP

This construct constitutively expresses Leaf Compost Cutinase (Basic part: BBa_K936000) linked with amilCP (Basic part: BBa_K592009). Cutinases are lypolytic/esterolytic enzymes that degrade and hydrolyze the ester bonds of cutin. The amilCP serves as a functional color reporter, allowing for convenient visible detection of the location of LC Cutinase. T--TAS_Taipei--K2921150.jpg Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 716
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None