Measurement

Part:BBa_K1678007

Designed by: Arthur Molcard   Group: iGEM15_Paris_Bettencourt   (2015-09-17)
Revision as of 07:25, 18 October 2019 by Wawapeng (Talk | contribs)


Vitamin B12 biosensor, composed by a cobalamin riboswitch in front of an mRFP1

This part can be used as a Vitamin B12 biosensor, we tested it for the AdoCobalamin form of B12 (AKA Coenzyme B12), but it might be used also to detect CyanoCobalamin, HydroxyCobalamin, MethylCobalamin according to the following publication : "A riboswitch sensor to determine vitamin B12 in fermented foods" Xuan Zhu 2015. We didn't tried this because we were out of time, anyway this would have to be characterized.

2019 OUC-China's characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 964
    Illegal AgeI site found at 1076
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna/riboswitch
Parameters
None