Composite

Part:BBa_K3017065

Designed by: Luk Hau Ching   Group: iGEM19_Hong_Kong_HKUST   (2019-10-17)
Revision as of 02:34, 18 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Construct for testing CRISPRi sgRNA suppression on fluorescence protein production

/

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3147
    Illegal PstI site found at 4569
    Illegal PstI site found at 4773
    Illegal PstI site found at 4803
    Illegal PstI site found at 6015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2131
    Illegal NheI site found at 2303
    Illegal NheI site found at 2326
    Illegal PstI site found at 3147
    Illegal PstI site found at 4569
    Illegal PstI site found at 4773
    Illegal PstI site found at 4803
    Illegal PstI site found at 6015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2608
    Illegal BamHI site found at 2070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3147
    Illegal PstI site found at 4569
    Illegal PstI site found at 4773
    Illegal PstI site found at 4803
    Illegal PstI site found at 6015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3147
    Illegal PstI site found at 4569
    Illegal PstI site found at 4773
    Illegal PstI site found at 4803
    Illegal PstI site found at 6015
    Illegal NgoMIV site found at 3435
    Illegal NgoMIV site found at 4539
    Illegal NgoMIV site found at 4612
    Illegal NgoMIV site found at 5097
    Illegal NgoMIV site found at 6006
    Illegal AgeI site found at 1905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705
    Illegal SapI site found at 1887


[edit]
Categories
Parameters
None