Coding

Part:BBa_K2926067

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)
Revision as of 22:44, 17 October 2019 by Jopgenoorth (Talk | contribs)

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Fusion protein of Opy2 (yeast), mCherry and pVIII (bacteriophage M13) with purification tag

The extracellular cysteine-rich domain of the membrane protein Opy2 from S. cerevisiae was n-terminally fused to the red fluorescing reporter protein mCherry. Additionally, the M13 bacteriophage major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy protein purification a hexahistidine tag was fused to the C-terminus of the protein.

Sequence and Features

Sequence was validated by Sanger sequencing.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None