Coding

Part:BBa_K2926056

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)
Revision as of 22:41, 17 October 2019 by Jopgenoorth (Talk | contribs)


Fusion protein of Opy2 from yeast, mCherry and pVIII from the bacteriophage M13

The extracellular domain of S. cerevisiaes membrane protein Opy2 was N-terminally fused to mCherry to enable protein visualization. Additionally, the major coat protein pVIII of the bacteriophage M13 was fused to the C-terminus of mCherry.

Sequence and Features

Sequence was validated by Sanger sequencing.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None