Plasmid

Part:BBa_K2908675:Design

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-16)
Revision as of 21:52, 17 October 2019 by Sqx98625 (Talk | contribs) (→‎References)

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s(ESR1)p-miR101spe-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture. T--CSU_CHINA--ggmodule2.png

Source

We designed it by ourselves and constructed this part by Golden gate.

References

Waclaw Szybalski”, Sun C. Kim”, Noaman Hasan” and Anna J. Podhajskab, Class IIS restriction enzymes, a review.1991, Gene, 100: 13-26.