DNA

Part:BBa_K3139001:Experience

Designed by: Jiapei Han   Group: iGEM19_NAU-CHINA   (2019-10-15)
Revision as of 20:49, 17 October 2019 by Thousandwinds (Talk | contribs) (→‎Applications of BBa_K3139001)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3139001

For characterizing other parts, we synthesized the RBS measurement device J23100 B0032 RFP (BBa_K528004). Firstly, we planned to improve the secretion of HlyA secretion system by inserting this part between the promoter and RBS. Unfortunately, the secretion system failed to come into effect, so we attempt to insert this sequence into BBa_K528004.

By measuring the florescent intensity of both bacterial liquids with this part inserted or not, we found that BBa_K528004 with the part inserted got the florescent intensity of almost 2-fold.

What interested us is, a former plan for improve the secretion of fusion protein finally got to be an improve possibly be used for many cases of protein synthesis. This part cuold be widely used as the effector gene - ribosomal protein S1 gene cannot get mutated as a lethal mutation.

Though the function of this part was basically proved, more sequences with different promoter, RBS and CDS are needed for further exploration.

Here's our result below.

<img src= "gold.png"/>

User Reviews

UNIQ2f1f7f35d705b0d5-partinfo-00000000-QINU UNIQ2f1f7f35d705b0d5-partinfo-00000001-QINU