Part:BBa_K3165001
CcaR v 2.0
CcaR produced naturally by the cyanobacterium Synechocystis sp.PCC6803 acts as a response regulator for the pCpcG2 promoter under the green light-inducible CcaSR system. In its phosphorylated form, CcaR activates the pCpcg2 promoter while the unphosphorylated form retards the activation of the same.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Biology
The CcaR protein acts as a cognate response regulator of the CpcG2 gene (under pCpcG2 promoter). In association with the multi-domain CcaS protein complex CcaR constitutes the green light-inducible CcaSR system. On illumination with green light, the CcaS protein gets autophosphorylated followed by phosphotransfer to CcaR through the histidine kinase domain. The phosphorylated form of CcaR activates the pCpcG2 promoter upregulating the expression of the gene downstream to the promoter. When acted upon by red light, the CcaR protein gets unphosphorylated, preventing the expression of the gene under the pCpcG2 promoter.
Usage
CcaR can be incorporated into a construct along with the CcaS system to replicate the green light-inducible CcaSR system. This part can also be used to characterise the functioning of the CcaSR system in relation to the pCpcG2 promoter at various frequencies and intensities of incident radiation.
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