Part:BBa_K2993000

Fusion protein of N-TEV-GSAT-ZincFingerArray1

This part contains the N-TEV split protein fused to a zincfinger array via a GSAT-linker.

This fusion protein should be used with its counterpart which contains the C-TEV protein fused to another zinc-finger array with a binding affinity for a different DNA sequence. On our iGEMteam_Rotterdam2019 webpage, you can read all about how to use this part properly.

This part is special in its own way because it actually is a fusion of one half of a protein to a whole array of zinc-finger proteins. Much like a multi fusion protein.

The full sequence of this composite is as follows: GAATTCGCGGCCGCTTCTAGAGCCTGCAATGACGGACGTATTCCTCTGGCCTCAACGCAAGATTGGCAGCTGAAACCGCCTTTCCAAAGTGA GTCCTTCGTCTATACGTTGCTTCTCAACGTGGCAAGTGACAGCACCACTGTGAACCAAACGTCCCGTTTCCACGTACCCAAGGGTGCCCAAT CTTGTATTAAGCattaaagaggagaaaaCTAGATGGCCGGCCACCACCATCACCATCACCATCACACCGGCggagaaagcttgtttaagggg ccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttgg tcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaaca ccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaa tttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactACCGGCGGAGGGTCGGCAGGAGGGAGTGGTTCCGG ATCTAGTGGTGGAAGCTCAGGAGCTAGTGGTACAGGCACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGCCTGGAGC CGGGTGAAAAACCGTACAAATGCCCTGAATGCGGTAAAAGCTTTAGTCGCAGCGACAAACTTGTGCGCCACCAGCGTACTCACACCGGCGAA AAACCATATAAATGCCCGGAGTGTGGCAAAAGCTTCAGTCGCGAAGATAACCTTCACACCCACCAGCGCACCCATACCGGCGAAAAGCCGTA TAAATGCCCAGAATGTGGCAAAAGCTTTAGCCATCGCACGACCCTGACCAACCACCAGCGCACCCACACCGGCGAGAAACCGTATAAATGTC CCGAGTGTGGAAAGTCTTTCTCTACAAGTGGCCATTTGGTGCGTCATCAACGTACTCACACCGGAGAAAAGCCGTACAAATGCCCAGAATGC GGTAAGTCCTTCTCTCGTGAGGACAACTTGCATACCCATCAACGCACGCACACGGGTGAGAAACCATATAAGTGCCCCGAGTGCGGAAAATC GTTTTCTCAGTCAAGTTCCTTAGTCCGCCATCAGCGCACACACACTGGGAAGAAGACTAGCTACTAGTAGCGGCCGCTGCAG

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 339