Part:BBa_K3013000

</html>LCI, polymer binding peptide LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI. We used this part to tag polypropylene and polystyrene with eGFP. ===Usage and Biology=== Therefore we made a fusion protein: eGFP - 17x helix - LCI. This Construct was expressed under a T7 promoter in E. coli BL21. The soluble protein fraction was purified and binding was tested in microtiter plates. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

===Functional Parameters===

biology	-NA-
function	-NA-
protein	-NA-

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