Coding

Part:BBa_K2984037

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 15:38, 17 October 2019 by Luise Pfannebecker (Talk | contribs)


L1c-Psad-PtxD-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (PtxD) which allows C.reinhardtii to grow on phosphite. The trancriptional unit starts with the PsaD promoter and Ends with the Rbcs2 terminator. The construct contains a B2-B2 linker which connects the PsaD promoter to PtxD.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1790
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii


[edit]
Categories
Parameters
chassisC.reinhardtii