Device

Part:BBa_K3226020

Designed by: Yuma matsumoto   Group: iGEM19_Botchan_Lab_Tokyo   (2019-10-16)
Revision as of 14:48, 17 October 2019 by Youma (Talk | contribs)


Add the his tag to BBa_K602008.

This part is an improved part of the device for expressing RecA made by Osaka (2011). His-tags were added so that the expressed RecA could be purified and recovered. By adding a histag, histidine residues having high affinity for metal ions form a cluster. This can be purified by Ni-NTA affinity chromatography using a carrier coordinated with Ni2 +, and the procedure is very simple, and results can be obtained within a day.

Usage As shown in the Long description, in order to examine how much RecA is produced by purifying and recovering the produced RecA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3396
    Illegal suffix found in sequence at 1348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3396
    Illegal SpeI site found at 1349
    Illegal PstI site found at 1363
    Illegal NotI site found at 1356
    Illegal NotI site found at 3402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3396
    Illegal BglII site found at 468
    Illegal XhoI site found at 2380
    Illegal XhoI site found at 3272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3396
    Illegal suffix found in sequence at 1349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3396
    Illegal XbaI site found at 3411
    Illegal SpeI site found at 1349
    Illegal PstI site found at 1363
    Illegal NgoMIV site found at 1142
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None