Part:BBa_K2984063
Phytobrick Level 2 backbone (with C.reinhardtii paromomycin marker)
Level 2 backbone
Usage and Biology
The backbone is desgined for MoClo level 2 cloning. Together with the L1a, L1b and L1c three different level 1 constrcuts can be inserted into the level 2 backbone. The backbone contains a transcriptional unit, which we call selction-cassette. This unit consists of the Psad promoter, the paromomycin resistance paroR and the Rbcs2 terminator. It is flanked by a N-terminal BamHI and a C-terminal XhoI restriction site to allow the exchange of the selection.cassette. Herefore our hygromycin construct or ptxD construct could be inserted instead. The selction-cassette benfits the transofrmation rate of Chlamydomonas since it makes co-transformations obsolete. It is not necessary to clone all three level 1 construct into the backbone. It could be redused to one or two using one of our Level 2 Linker
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 1121
Illegal XhoI site found at 3012 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal AgeI site found at 189
Illegal AgeI site found at 301 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
| None |