Composite

Part:BBa_K2924034

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-16)
Revision as of 14:16, 17 October 2019 by AndreasN (Talk | contribs)


Pcpc560 + α-s1-casein + T1/T7 Terminator

Strong constitutive cyanobacterial promoter Pcpc560 expressing a-s1-casein with the T1/T7 double terminator. ===Usage and Biology===

The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from E. coli to cyanobacteria and other microorganisms1. After testing the strength of the promoter with a fluorescent reporter mVenus, it was used to express a protein from cow’s milk, a-s1-casein in Synechocystis sp. PCC 6803.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


</html>

[edit]
Categories
Parameters
None