Coding
YFP B4-B4

Part:BBa_K2984024

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-15)
Revision as of 14:11, 17 October 2019 by Chlamy Dima (Talk | contribs)


YFP - Yellow fluorescent protein for detection B4-B4

This part is a part of the Chlamy-HUB-Collection. For in vivo detection of proteins in C. reinhardtii or its surroundings. The green fluorescent protein variant carries amino acid substitutions that shift the protein to which it is bound to an emission peak at 530 nm seen as yellow (Ormo et al., 1996). For detection of fluorescence it is not necessary to process a sample nor adding a substance to make it visible making it safe for C. reinhardtii as it can be detected with a fluorescence microscope.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None