Part:BBa_K2984035

L1c-Psad-PETase-YFP-His-RbcS2

This vector is a part of the Chlamy-HUB-Collection. L1c plasmid containing PETase enzyme, fused to mVenus fluorescent marker for fluorecence meausrements of PETase concentration and His-tag for purification. The parts are flanked by the PsaD promoter and the Rbcs2 terminator. The part has a B2-B2 linker connecting the PsaD promoter and the PEtase. The construct is desgined to measure the expression level of PETase.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1705
  Illegal PstI site found at 1919
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1244
  Illegal PstI site found at 1705
  Illegal PstI site found at 1919
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  Illegal XhoI site found at 968
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1705
  Illegal PstI site found at 1919
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1705
  Illegal PstI site found at 1919
  Illegal NgoMIV site found at 2396
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii