Regulatory

Part:BBa_K2771000

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-06)
Revision as of 11:40, 17 October 2019 by Ruizhou (Talk | contribs) (→‎XHD-WS-Wuhan-A characterization)


Modified pLux

Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.



Usage and Biology

XHD-WS-Wuhan-A characterization

We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for part K2771000, thus making a valuable characterization for this part.

Plux is a promoter which responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). In order to test this promoter function. We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for this part K2771000, thus making a valuable characterization for this part.

Experiment Design and Measurement Methods

We cloned LuxR, lux promoter and GFP into psB1C3 vector, and constructed ppSB1C3-LuxR-Plux-GFP plasmid. gene circuit as Fig1.:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

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Categories
Parameters
None