Regulatory

Part:BBa_K2771000

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-06)
Revision as of 11:27, 17 October 2019 by Ruizhou (Talk | contribs)


Modified pLux

Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.



Usage and Biology

The expression of miR-196a was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None