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Team NTHU Taiwan 2019: Effects and inducing of fatty acid to E.coli and Relative Fluorescence to Anderson Promoter

Overview

From previous study, NTU-Taida found that pFadBA can be induce by adding fatty acid. We’d like to understand more about this promoter by answering the following questions:
**Does fatty acid affect the growth of cell? **Will higher fatty acid concentration induce more fluorescence? **How strong is the promoter pFadBA?
====Effect of Fatty acid to E. coli==== We discuss the relationship between fatty acid and the growth of E. coli to check suitable conditions for future experiments. First, we check whether fatty acid affect our experiments. Second, we choose the best concentration for future experiments.
In general, relative cell concentration does not change very much. It indicates that under 0.4mM and 3.125mM fatty acid, E. coli might grow better than control. However, when the concentration is 25mM, it inhibits growth of E. coli. We can notice that low concentration of fatty acid is somehow benefit for cell growth. Therefore, we choose concentration lower than 10 mM as our highest concentration to induce the promoter in the following experiments.

https://2019.igem.org/wiki/images/a/a4/T--NTHU_Taiwan--characterization1.png

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