Coding
Part:BBa_K2984043
Designed by: Dimitri Schumacher Group: iGEM19_Humboldt_Berlin (2019-10-16)
Revision as of 10:41, 17 October 2019 by Chlamy Dima (Talk | contribs)
L1c-PsaD-MHETase-3xHA-RbcS2
This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the MHETase enzyme, kindly provided by the iGEM TU Kaiserslautern 2019 team, a 3xHA-tag, and the Rbcs2 terminator. The part can be used to express the MHETase enzyme in C. reinhardtii. The 3xHA tag can be used to detect and purify the MHETase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086
Illegal NotI site found at 1944 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2854
Illegal BamHI site found at 4 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1609
Illegal PstI site found at 1933
Illegal PstI site found at 2276
Illegal PstI site found at 3086
Illegal NgoMIV site found at 1542
Illegal NgoMIV site found at 2003
Illegal NgoMIV site found at 2033
Illegal NgoMIV site found at 2348
Illegal NgoMIV site found at 2366
Illegal NgoMIV site found at 2402
Illegal NgoMIV site found at 3045 - 1000COMPATIBLE WITH RFC[1000]
Characterization
[edit]
Categories
Parameters
chassis | C. reinhardtii |
emission | 528nm |
excitation | 515 nm |
protein | MHETase |