Coding

Part:BBa_K2984030

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 09:19, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-Psad-ARS-YFP-SP20-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. L1 construc containg Arylsulfatase secretion Signal ARS, mVenus fluorescent marker, SP20 glycomodule. These parts are flanked by the PsaD promoter and the Rbcs2 terminator. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]



[edit]
Categories
Parameters
None