Composite

Part:BBa_K2921550

Designed by: Allison Kuo   Group: iGEM19_TAS_Taipei   (2019-10-14)
Revision as of 09:07, 17 October 2019 by Allishannn (Talk | contribs)



Metallothionein + EAAAK-linker + mRFP

This construct constitutively expresses a colored metal-binding fusion protein: metallothionein (Basic part: BBa_K1460002) linked with mRFP (Basic part: BBa_E1010). Metallothionein is a metal-binding protein that can tightly chelate heavy metal ions by forming a strong coordination bond. The mRFP serves as a functional color reporter, allowing for the convenient visible or UV detection of the location of metallothionein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1531
    Illegal AgeI site found at 1643
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 118


[edit]
Categories
Parameters
None