Composite

Part:BBa_K3160009

Designed by: chi xu   Group: iGEM19_XHD-WS-Wuhan-A   (2019-10-14)
Revision as of 08:58, 17 October 2019 by Lavenderxc (Talk | contribs)


pEGFP-miR-196a sensor

The expression of miR-196a was found to be significantly associated with all tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites tomiR-196a1, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP-miR-196a sensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None