Coding

Part:BBa_K2993001

Designed by: Anass Fendrhoussi   Group: iGEM19_Rotterdam_HR   (2019-10-12)
Revision as of 08:32, 17 October 2019 by Anass (Talk | contribs)


ZincFingerArray1

This part encodes for the six zinc-finger arrays which can bind to a specific DNA sequence encoded in our DNA-bridges. This protein sequence can be used in other projects to connect other proteins with DNA/RNA sequences such as we were planning to do in our own project.

The zinc finger arrays were designed with the help of www.zincfingertools.com. There we made sure that the DNA binding sequence doesn't surpasses more than two matches with the genome of E.coli itself so that we do not get any fals positive binding reactions.

The sequence of this part is: CTGGAGCCGGGTGAAAAACCGTACAAATGCCCTGAATGCGGTAAAAGCTTTAGTCGCAGCGACAAACTTGTGCGCCACCAGCGTACTCACACCGGCGAAAAACCATATAAATGCCCGGAGTG TGGCAAAAGCTTCAGTCGCGAAGATAACCTTCACACCCACCAGCGCACCCATACCGGCGAAAAGCCGTATAAATGCCCAGAATGTGGCAAAAGCTTTAGCCATCGCACGACCCTGACCAACCA CCAGCGCACCCACACCGGCGAGAAACCGTATAAATGTCCCGAGTGTGGAAAGTCTTTCTCTACAAGTGGCCATTTGGTGCGTCATCAACGTACTCACACCGGAGAAAAGCCGTACAAATGCCC AGAATGCGGTAAGTCCTTCTCTCGTGAGGACAACTTGCATACCCATCAACGCACGCACACGGGTGAGAAACCATATAAGTGCCCCGAGTGCGGAAAATCGTTTTCTCAGTCAAGTTCCTTAGTC CGCCATCAGCGCACACACACTGGGAAGAAGACTAGC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None