Part:BBa_K2660000

Penetratin, cell-penetrating peptide

Penetratin is a 16 residue cell-penetrating peptide, RQIKIWFQNRRMKWKK, derived from the homeodomain of the Drosophila Antennapedia transcription factor. Cell-penetrating peptides are small peptides that improve intracellular delivery of various molecules in cells and tissues in vivo and in vitro and represent an important tool to deliver therapeutics.

The translocation of penetratin is influenced by hydrophobic and electrostatic properties. It was shown that the increase of hydrophobicity of the protein of interest and penetratin induces a transient destabilization of the plasma membrane and this is crucial for the protein/peptide translocation.

To use this part, add the sequence to the N-terminal sequence of your protein of interest.

Biosafety

ogether with professional safety officials, Team iGEM Tuebingen 2019 has evaluated the biosafety of cell penetrating peptides and has come to the conclusion that cell penetrating peptides are to be classified as BSL1.

References

1. Dupont E., Prochiantz A., Joliot A. (2011) Penetratin Story: An Overview. In: Langel Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology (Methods and Protocols), vol 683. Humana Press

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]