DNA

Part:BBa_K2940010:Design

Designed by: Ping CHANG   Group: iGEM19_Edinburgh_OG   (2019-10-16)
Revision as of 04:01, 17 October 2019 by Registry (Talk | contribs)

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Immobilised dye-degrading peroxidase: Dyp (without signal peptides)+ Cytochrome b5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 995
    Illegal SapI site found at 425
    Illegal SapI site found at 783
    Illegal SapI.rc site found at 939


Design Notes

This part is designed for the DyP-Cytb5 expression. So the signal peptides were removed from the original coding sequence to make sure DyP can anchor with Cytb5. The strong promoter and RBS were introduced to make the DNA construct. It was cloned into pSB1C3 using classcial cloning and was verified using ABTS assay


Source

NC_000964.3


References