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DNA
Part:BBa_K2940010:Design
Designed by: Ping CHANG Group: iGEM19_Edinburgh_OG (2019-10-16)
Immobilised dye-degrading peroxidase: Dyp (without signal peptides)+ Cytochrome b5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 995
Illegal SapI site found at 425
Illegal SapI site found at 783
Illegal SapI.rc site found at 939
Design Notes
This part is designed for the DyP-Cytb5 expression. So the signal peptides were removed from the original coding sequence to make sure DyP can anchor with Cytb5. The strong promoter and RBS were introduced to make the DNA construct. It was cloned into pSB1C3 using classcial cloning and was verified using ABTS assay
Source
NC_000964.3