Part:BBa_K3292001
BBa_K3292001 sfGFP cassette
This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after adding its nucleotide sequence next to the sfGFP.
In our case, we decided to add the coding sequence of sialidase from M. viridifaciens. The CDS of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.
Sequence and Features
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