Coding
Part:BBa_K2989008
Designed by: Meng Chen Group: iGEM19_Nanjing_NFLS (2019-10-16)
DNLS-NS5B
RNA-dependent RNA polymerase (RdRP) is an enzyme that replicates RNA using RNA as a template. This enzyme is important for viruses that lack DNA components and synthesize new RNA genomes through RNA. Because it has no functional similarities in human cells, this makes the inhibitor of NS5B highly selective, so it can be used as an excellent antiviral drug target. In DNLS-NS5B, we added two repeating nuclear localization sequences to help localize proteins into the nucleus
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 492
Illegal BglII site found at 1340 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 537
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Categories
Parameters
None |