Coding

Part:BBa_K2010999

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-17)
Revision as of 02:59, 17 October 2019 by VSajtovich (Talk | contribs)


PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

ATGAATTTTCCTCGCGCTTCACGACTGATGCAGGCTGCCGTTCTTGGAGGGCTGATGGCCGTTAGCGCGGCCGCCACCGCTCAGACAAATCCCTACGCCCGCGGTCCGAATCCGACAGCCGCCAGTTTGGAAGCGAGCGCTGGTCCATTCACCGTTCGCTCCTTTACCGTGAGTAGACCGAGCGGTTATGGCGCTGGCACCGTTTACTATCCAACAAATGCTGGGGGTACCGTGGGCGCCATAGCCATAGTTCCCGGGTATACGGCACGGCAGTCATCAATTAAATGGTGGGGACCGCGTCTGGCATCCCACGGTTTCGTAGTAATTACAATTGACACAAATTCCACGTTAGACCAGCCATCAAGTCGGAGTTCGCAACAAATGGCCGCGCTGCGCCAGGTGGCGTCGTTAAACGGTACAAGTAGCAGCCCGATTTACGGAAAGGTCGATACCGCTCGTATGGGTGTTATGGGGTGGAGTATGGGAGGTGGAGGCTCCCTGATCTCTGCTGCTAACAACCCTTCGCTGAAAGCAGCGGCGCCTCAAGCACCATGGGATTCTTCGACAAATTTTAGTTCTGTAACTGTGCCCACGCTGATCTTCGCATGTGAAAACGATAGTATAGCCCCGGTCAACTCTTCAGCACTTCCTATCTATGATTCTATGTCACGCAACGCTAAGCAGTTTCTCGAAATTAATGGTGGCTCACATTCCTGTGCGAATAGCGGCAATTCTAACCAAGCATTAATCGGAAAAAAAGGCGTTGCATGGATGAAACGTTTTATGGACAATGATACTAGGTATTCTACTTTTGCCTGCGAGAACCCGAATAGCACCAGAGTGTCTGATTTTCGTACAGCGAATTGCAGCTGA


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None