Coding

Part:BBa_K2910003

Designed by: Dimitrije Ratkov   Group: iGEM19_Toronto   (2019-10-16)
Revision as of 02:39, 17 October 2019 by Registry (Talk | contribs)

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IsPETase-T88F-I208Y-S238F-S245Y-R280A with C-terminal Hexahistidine Tag

This part encodes a poly(ethylene terephthalate) hydrolase with several mutations returned by FuncLib to increase its catalytic activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None