Coding

Part:BBa_K2960007:Design

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 01:32, 17 October 2019 by Aemetzloff (Talk | contribs) (→‎Design Notes)


The mlrB serine protease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1124


Design Notes

The mlrB gene sequence has been codon-optimized for expression in E. coli.

The construct was designed to be RFC[10] compatible.

Source

Sphingopyxis sp. genomic sequence. European Nucleotide Archive. (2015 April 16). Received from https://www.ebi.ac.uk/ena/data/view/KR150744

References