Coding

Part:BBa_K2960002:Design

Designed by: Michelle Croen   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 01:27, 17 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


mlrB with TorA tat Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1297


Design Notes

We have designed this part to be RFC[10] compatible. We also optimized codons for expression in E. coli.


Source

European Nucleotide Archive. (2015, April 16). Retrieved from https://www.ebi.ac.uk/ena/data/view/KR150744.


References